| Place | Lane | Name | School | Time |
| 1 | 4 | Kelli Okuji | Robert Lewis Stevenson | 45.54 |
| 2 | 3 | Dahlys Marshall | Valley Christian High Sch | 45.56 |
| 3 | 6 | Sharrell Smith | Silver Creek | 45.93 |
| 4 | 5 | Stacie Boyle | Cupertino High School | 45.95 |
| 5 | 1 | Keriann Aronson | Mills High School | 46.34 |
| 6 | 7 | Jennifer Connet | Menlo Atherton High Sch | 46.92 |
| 7 | 2 | Meghan Przybyla | Palo Alto High School | 47.12 |
| 8 | 8 | Christina Agoff | Aragon High School | 47.64 |