| Place | Lane | Name | School | Time |
| 1 | 5 | Sharrell Smith | Silver Creek | 47.10 |
| 2 | 4 | Christine Reed | Los Gatos High School | 47.56 |
| 3 | 3 | Christina Nguyen | St. Francis High School | 47.81 |
| 4 | 6 | Elissa Chandler | Palo Alto High School | 47.98 |
| 5 | 7 | Stephanie Christie | Salinas High School | 48.38 |
| 6 | 2 | Erica Buchinski | Cupertino High School | 48.96 |
| 7 | 1 | Kim O'Donnell | Menlo Atherton High Sch. | 49.55 |
| 8 | 8 | Irene Chen | Piedmont Hills | 52.69 |