| Place | Lane | Name | School | Time | Heat | Heat Place |
| 1 | 4 | Libby Jenke | Menlo School | 56.09 | 2 | 1 |
| 2 | 4 | Mia Lattanzi | Palo Alto High School | 57.35 | 1 | 1 |
| 3 | 4 | Marshay Brown | Mt. Pleasant | 57.51 | 5 | 1 |
| 4 | 4 | Bria Nelson | Los Altos High School | 57.78 | 3 | 1 |
| 5 | 4 | Samantha Williams | St. Francis High School | 57.82 | 4 | 1 |
| 6 | 5 | Hayley Huxtable | Scotts Valley High School | 58.08 | 5 | 2 |
| 7 | 3 | Diedre Pettigrue | Mt. Pleasant | 58.16 | 4 | 2 |
| 8 | 3 | Lakesha Adams | Monterey High School | 58.24 | 3 | 2 |
| 9 | 5 | Brieanna Wright | Scotts Valley High School | 58.44 | 3 | 3 |
| 10 | 6 | Jeanell English | Mitty High School | 58.53 | 5 | 3 |
| 11 | 2 | Tannia Hernandez | North Salinas High School | 58.56 | 2 | 2 |
| 12 | 3 | Sammi Moser | Los Gatos High School | 58.75 | 1 | 2 |
| 13 | 5 | Kelly Long | San Lorenzo Valley HS | 58.88 | 4 | 3 |
| 14 | 6 | Annelise Weber | San Mateo High School | 58.94 | 2 | 3 |
| 15 | 3 | Camille Brown | Pioneer | 59.12 | 2 | 4 |
| 16 | 5 | Stacie Boyle | Cupertino High School | 59.14 | 1 | 3 |
| 17 | 6 | Kristina Llorin | Piedmont Hills | 59.26 | 4 | 4 |
| 18 | 3 | Krysta McGowan | Mt. Pleasant | 59.28 | 5 | 4 |
| 19 | 6 | Eileen Nguyen | Milpitas High School | 59.30 | 3 | 4 |
| 20 | 5 | Michaela King | Westmoor High School | 59.66 | 2 | 5 |
| 21 | 2 | Emily Bruton | Redwood Christian School | 59.72 | 3 | 5 |
| 22 | 2 | Vanessa Block | Santa Catalina High Schoo | 59.95 | 1 | 4 |
| 23 | 2 | Kaitlin McGhee | Menlo Atherton High Sch. | 1:00.47 | 4 | 5 |
| 24 | 6 | Nicole Sabes | Saratoga High School | 1:00.88 | 1 | 5 |
| 25 | 2 | Kirstin McGhee | Menlo Atherton High Sch. | 1:01.88 | 5 | 5 |
| 26 | 7 | Kayla Souza | Salinas High School | 1:01.89 | 3 | 6 |
| 27 | 7 | Angela Pizzella | Willow Glen | 1:01.95 | 5 | 6 |
| 28 | 7 | Lynn Larkin | Notre Dame Salinas HS | 1:01.98 | 4 | 6 |
| 29 | 7 | Michele Mitchell | Leigh | 1:03.53 | 2 | 6 |
| 30 | 1 | Saira Toscano | James Lick | 1:04.39 | 2 | 7 |
| 31 | 7 | Danielle Ott | Leland | 1:04.92 | 1 | 6 |