| Place | Lane | Name | School | Time | Qual Mark |
| 1 | 4 | Alyssa Biondi (9) | LA | 12.88 | 00:13.02 |
| 2 | 5 | Tatiana Thomas (10) | LA | 13.11 | 00:13.29 |
| 3 | 3 | Deanna Brown (10) | LA | 13.53 | 00:13.43 |
| 4 | 2 | Rebecca Paley-Williams (9) | LG | 13.88 | 00:13.84 |
| 5 | 6 | Tiffany Stone (9) | Gunn | 13.90 | 00:13.83 |
| 6 | 1 | Mai-Lee Hung (9) | Paly | 13.98 | 00:14.13 |
| 7 | 7 | Krystle Peterson (10) | Wil | 14.03 | 00:14.11 |
| 8 | 8 | Emily Lin (9) | Lyn | 14.63 | 00:14.36 |