| Place | Lane | Name | School | Time |
| 1 | 5 | Riordan High School | ARIO A | 42.04 |
| 2 | 4 | Mitty High School | AMIT A | 42.53 |
| 3 | 6 | Monterey High School | MONT A | 42.66 |
| 4 | 7 | St. Francis High School | STFS A | 42.87 |
| 5 | 2 | Wilcox High School | WILC A | 43.01 |
| 6 | 8 | Evergreen Valley | EVGV A | 43.26 |
| 7 | 3 | Lynbrook High School | LYNB A | 43.45 |
| 8 | 1 | San Benito Hollister HS | SABE A | 43.97 |