| Place | Lane | Name | School | Time | Qual Mark |
| 1 | 4 | Ashley Benson (12) | LG | 2:26.37 | 02:26.90 |
| 2 | 3 | Callie Mulgannon (9) | LG | 2:27.84 | 02:26.60 |
| 3 | 2 | Daphne Owen (12) | LA | 2:29.59 | 02:26.00 |
| 4 | 7 | Alicia Ivanhoe (10) | Paly | 2:30.24 | 02:29.00 |
| 5 | 1 | Kelsey Feeley (9) | Gunn | 2:30.29 | 02:25.65 |
| 6 | 9 | Sarah Flamm (10) | Paly | 2:30.70 | 02:32.00 |
| 7 | 6 | Kelly Garton (9) | LG | 2:32.99 | 02:28.30 |
| 8 | 5 | Samantha Peppard (10) | LG | 2:33.89 | 02:28.27 |
| 9 | 10 | Peiley Lau (9) | Wil | 2:33.95 | 02:34.10 |
| 10 | 8 | Irene Graham (10) | Gunn | 2:34.78 | 02:31.24 |
| 11 | 11 | Kelly Duong (12) | Milp | 2:39.76 | 02:40.00 |